Isolation and characterization of more efficient amylolytic fungal species from the forest soils by serial dilution method was followed for the production of industrially important fungal amylases. Qualitative screening yields 15 fungal species, among them more efficient six fungal strains were selected based on the rate of zone of clearance on the starch agar plates by starch hydrolysis test. Production of amylase was done by submerged fermentation (SmF), Production medium supplemented with 2% (w/v) soluble starch incubated under shake culture at a temperature of 28±1ºC, pH-7.0 for 7 days. Maximum amylolytic activity was observed with crude enzyme at 3rd day of incubation by Penicillium sp. (0.87±0.05 U/mL) followed by Penicillium chrysogenum (0.69±0.05 U/mL), Aspergillus candidus (0.67±0.03 U/mL), Aspergillus fumigatus (0.066±0.06 U/mL) and at 7th day of incubation was by Penicillium sp. (1.13±0.03 U/mL) followed by Penicillium chrysogenum (1.12±0.004 U/mL) than the other isolates. Partially purified amylolytic enzyme activities were maximum at 7th day of incubation by Penicillium sp. (0.67±0.03 U/mL) followed by Aspergillus flavus (0.63±0.01 U/mL) and Aspergillus fumigatus (0.63±0.03 U/mL). Soluble crude protein content was maximum by Penicillium chrysogenum (150±3 µg/mL), Aspergillus fumigatus (90±4 µg/mL) and Penicillium sp. (88±4 µg/mL) and partially purified protein content was maximum by Aspergillus flavus (118±3.6 µg/mL), Penicillium sp. (90±2 µg/mL) and Aspergillus fumigatus (88±4.6 µg/mL) at 7th day of incubation.
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